Zymo-Seq WGBS Library Kit

Product Description

The Zymo-Seq WGBS Library Kit is designed for whole-genome bisulfite sequencing (WGBS) library preparation. It includes all necessary reagents and components for efficient and reliable DNA methylation analysis. The kit is suitable for preparing libraries from various DNA sources and ensures high conversion rates and library complexity.

Technical Details

The Zymo-Seq WGBS Library Kit from Zymo Research is designed for whole-genome bisulfite sequencing (WGBS) library preparation. The kit includes reagents and consumables necessary for the preparation of high-quality WGBS libraries. This includes bisulfite conversion reagents, polymerase chain reaction (PCR) reagents, primers, and buffer solutions. The kit is optimized for both high efficiency and reduced bias in the sequencing of methylated and unmethylated cytosines.

Usage Instructions

1. Start by preparing your DNA sample according to the kit instructions. Ensure the DNA is of high quality and free from contaminants. 2. Perform bisulfite conversion using the provided reagents to convert unmethylated cytosines to uracil while leaving methylated cytosines unchanged. 3. Purify the bisulfite-converted DNA using the purification columns provided in the kit. 4. Amplify the bisulfite-converted DNA using the provided PCR reagents and primers. 5. Purify the amplified DNA to obtain the final WGBS library. 6. Quantify and assess the quality of the library using appropriate methods such as qPCR or Bioanalyzer. 7. The prepared library is now ready for sequencing on a next-generation sequencing (NGS) platform. Follow the sequencing platform's instructions for loading and running the library.

Storage Conditions

Store the Zymo-Seq WGBS Library Kit at -20°C. Ensure that the kit components are kept in their original packaging to maintain stability and avoid contamination. Avoid repeated freeze-thaw cycles.

Handling Instructions

Handle all kit components with care to avoid contamination. Use sterile pipette tips and tubes. Follow the protocol provided by the manufacturer precisely to ensure optimal results. Wear appropriate personal protective equipment (PPE) such as gloves and lab coat when handling reagents.

Packaging Details

Packaging Type: Kit

Packaging Condition: New

Additional Notes

This product is intended for research use only and not for use in diagnostic procedures. Store the kit components as directed in the accompanying manual, typically at -20°C for long-term storage. Ensure all reagents are at room temperature before use to ensure optimal performance.