Folin & Ciocalteu's phenol reagent

Product Description

Used for the determination of total protein in biological samples. Contains less than 2% water.

Technical Details

This is a Sigma-Aldrich product, specifically a reagent used for the Folin & Ciocalteu's phenol reagent assay. It is typically used in biochemical experiments to measure protein concentrations by reacting with phenolic compounds. The reagent is sensitive to phenolic groups, resulting in a colorimetric change that can be measured spectrophotometrically.

Usage Instructions

To use the Folin & Ciocalteu's phenol reagent, prepare your protein sample and dilute it appropriately. Mix the sample with an alkaline solution, typically sodium carbonate. Add the Folin-Ciocalteu reagent and incubate the mixture at room temperature for a specific time, usually around 30 minutes. Measure the absorbance using a spectrophotometer at a wavelength of 750 nm to determine protein concentration. Ensure to follow safety guidelines, such as wearing gloves and goggles, as the reagent may be hazardous.

Storage Conditions

Store in a cool, dry place away from incompatible substances. Keep the container tightly closed when not in use.

Handling Instructions

Use personal protective equipment as required. Avoid contact with skin and eyes. Handle under a chemical fume hood to avoid inhalation of vapors.

Packaging Details

Packaging Type: Original

Packaging Condition: Sealed

Additional Notes

This is a reagent for laboratory use only. Not for human or animal consumption.